Development of Microbial-Human Enterocyte Interaction: Cholera Toxin
نویسندگان
چکیده
منابع مشابه
Interaction of Cholera Toxin and Toxin Derivatives with Lymphocytes
The interaction of cholera toxin and a number of toxin derivatives, containing different proportions of light and heavy toxin-composing subunits (L and H), with mouse lymphocytes was studied. Experiments with [(125)I]toxin showed that a single cell can rapidly, within minutes, bind up to 40,000 molecules of toxin, the association constant was estimated to 7 +/- 4 x 10(8) liters/mol, and binding...
متن کاملCholera Toxin
Vibrio cholerae, the causative agent of cholera, requires two coordinately regulated factors for full virulence: cholera toxin (CT), a potent enterotoxin, and toxin-coregulated pili (TCP), surface organelles required for intestinal colonization. The structural genes for CT are shown here to be encoded by a filamentous bacteriophage (designated CTXCP), which is related to coliphage Ml 3. The CTX...
متن کاملDevelopment of a purified cholera toxoid. I. Purification of toxin.
The enterotoxin from Vibrio cholerae is selectively concentrated from cell-free culture supernatant by co-precipitation with hexametaphosphate and is further purified by adsorption on aluminum hydroxide powder. The bulk of residual somatic antigen becomes insoluble upon lyophilization of the toxin preparation and is removed by centrifugation of the rehydrated material. Other contaminants are el...
متن کاملEffect of cholera toxin on the human jejunum.
In order to develop a model for secretory diarrhoea and to confirm the in vitro effects of cholera toxin in man in vivo the effect of intrajejunally administered cholera toxin was investigated in healthy volunteers. An intestinal perfusion technique with an occluding balloon proximal to the infusion site was used. The jejunum was perfused under steady state conditions with a plasma like electro...
متن کاملEndocytosis of cholera toxin by human enterocytes is developmentally regulated.
Many secretory diarrheas including cholera are more prevalent and fulminant in young infants than in older children and adults. Cholera toxin (CT) elicits a cAMP-dependent chloride secretory response in intestinal epithelia, which accounts for the fundamental pathogenesis of this toxigenic diarrhea. We have previously reported that the action of this bacterial enterotoxin is excessive in immatu...
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ژورنال
عنوان ژورنال: Pediatric Research
سال: 2003
ISSN: 0031-3998,1530-0447
DOI: 10.1203/01.pdr.0000074974.21797.83